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KMID : 1094720000050040219
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 4 p.219 ~ p.226
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Production of therapeutic glycoproteins through the engineering of glycosylation pathway in yeast
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Roy Samir Kumar
Chiba Yasunori
Jigami Yoshifumi
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Abstract
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The application of recombinant DNA technology to restructure metabolic networks can change metabolite and protein products by altering the biosynthetic pathways in an organism. Although some success has been achieved, a more detailed and thorough investigation of this approach is certainly warranted since it is clear that such methods hold great potential based on the encouraging results obtained so far. In last decade, there have been tremendous advances in the field of glycobiology and the stage has been set for the biotechnological production of glycoproteins for therapeutic use. Today glycoproteins are one of the most important groups of pharmaceutical products. In this study the attempt was made to focus on identifying technologies that may have general application for modifying glycosylation pathway of the yeast cells in order to produce glycoproteins of therapeutic use. The carbohydrates of therapeutic recombinant glycoproteins play very important roles in determining their pharmacokinetic properties. A number of biological interactions and biological functions mediated by glycans are also being targeted for therapeutic manipulationin vivo. For a commercially viable production of therapeutic glycoproteins a metabolic engineering of a host cell is yet to be established.
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KEYWORD
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Metabolic engineering, Saccharomyces cerevisiae, glycoprotein, sugar nucleotide transporter, glycosyltransferase, glycosidase
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