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KMID : 1094720000050040253
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 4 p.253 ~ p.262
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Current progress in the analysis of transcriptional regulation in the industrially valuable microorganism Aspergillus oryzae
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Nakajima Keiichi
Sano Motoaki
Machida Masayuki
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Abstract
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Aspergillus oryzae is considered to be an attractive host for heterologous protein production because of its safety and ability to secrete large amounts of proteins. In order to obtain high productivity, thus far promoters of amylases have been most widely used inA. oryzae. Recent progress in cloning and expression analysis, including EST sequencing, revealed that glycolytic genes represent some of those most strongly expressed inA. oryzae. Therefore, promoters of glycolytic genes could be important alternatives to promoters of amylases because lower amounts of proteases are produced in the presence of glucose. SeveralA. oryzae transcription factors responsible for the induction and/or maximum expression of many industrially important genes encoding amylases and proteases have been cloned and characterized. In addition to the transcriptional regulatory factors, the gene encoding the largest subunit of RNA polymerase II, constituting the basic transcription machinery, has also been cloned fromA. orzae. This recently acquired understanding of the details of transcriptional regulatory mechanisms and factors will facilitate engineering flexible controls for the expression of proteins important for the fermentation industries.
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KEYWORD
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Aspergillus oryzae, transcriptional regulation, transcriptional regulatory factor, promoter
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