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KMID : 1094720000050050379
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 5 p.379 ~ p.381
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Fermentative production of succinic acid from glucose and corn steep liquor by Anaerobiospirillum succiniciproducens
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Lee Pyung-Cheon
Lee Woo-Gi
Lee Sang-Yup
Chang Ho-Nam
Chang Yong-Keun
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Abstract
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Anaerobiospirillum succiniciproducens requires expensive complex nitrogen sources such as yeast extract and polypeptone for its growth and succinic acid production. It was found thatA. succiniciproducens was able to grow in a minimal medium containing glucose when supplemented with corn steep liquor (CSL) as the sole complex nitrogen source. The concentration of CSL had a significant effect on the glucose consumption byA. succiniciproducens. When 10?15 g/L of CSL was supplemented, cells were grown to an OD660 of 3.5 and produced 17.8 g/L succinic acid with 20 g/L glucose. These results are similar to those obtained by supplementing yeast extract and polypeptone, thereby suggesting that succinic acid can be produced more economically using glucose and CSL.
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KEYWORD
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succinic acid, corn steep liquor, fermentation, Anaerobiospirillum succiniciproducens
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