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KMID : 1094720060110040299
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Biotechnology and Bioprocess Engineering
2006 Volume.11 No. 4 p.299 ~ p.305
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Asymmetric synthesis of unnaturall -amino acids using thermophilic aromaticl -amino acid transaminase
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Cho Byung-Kwan
Seo Joo-Hyun
Kim Ju-Han
Lee Chang-Soo
Kim Byung-Gee
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Abstract
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Aromaticl-amino acid transaminase is an enzyme that is able to transfer the amino group froml-glutamate to unnatural aromatic α-keto acids to generate α-ketoglutarate and unnatural aromaticl-amino acids, respectively. Enrichment culture was used to isolate thermophilicBacillus sp. T30 expressing this enzyme for use in the synthesis of unnaturall-amino acids. The asymmetric syntheses ofl-homophenylalanine andl-phenylglycine resulted in conversion yields of >95% and >93% from 150 mM 2-oxo-4-phenylbutyrate and phenylglyoxylate, respectively, usingl-glutamate as an amino donor at 60°C. Synthesizedl-homophenylalanine andl-phenylglycine were optically pure (>99% enantiomeric excess) and continuously pre-cipitated in the reaction solution due to their low solubility at the given reaction pH. While the solubility of the α-keto acid substrates is dependent on temperature, the solubility of the unnaturall-amino acid products is dependent on the reaction pH. As the solubility difference between substrate and product at the given reaction pH is therefore larger at higher temperature, the thermophilic transaminase was successfully used to shift the reaction equilibrium toward rapid product formation.
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KEYWORD
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thermophilic enzyme, transaminase, unnatural amino acids, asymmetric synthesis, equilibrium-shift, enrichment culture
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