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KMID : 1094720060110040364
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Biotechnology and Bioprocess Engineering
2006 Volume.11 No. 4 p.364 ~ p.367
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Effects of silkworm hemolymph and cartilage-specific extracellular matrices on chondrocytes and periosteum-derived progenitor cells
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Shin Hyun-Chong
Kim Dong-Il
Choi Yong-Soo
Lim Sang-Min
Lee Chang-Woo
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Abstract
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In order to enhance the repair of defects in articular cartilage via cell therapy with autologous chondrocytes, as well as with periosteum-derived progenitor cells (PDPCs), silkworm hemolymph (SH) and a variety of cartilage-specific extracellular matrices (ECMs) including type II collagen, proline, chondroitin 4-sulfate, and chondroitin 6-sulfate were assessed with regard to their efficacy as media supplements. SH, a known anti-apoptotic agent, was found to enhance cell growth, as was shown by the results of a 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. According to the results of reverse transcriptase polymerase chain reaction (RT-PCR) analyses, the cartilage-specific ECMs were found to stimulate the expression of hyaline cartilage-specific genes, most notably type II collagen and Sox9, in monolayer cultures of PDPCs.
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KEYWORD
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chondrocytes, periosteum-derived progenitor, silkworm hemolymph, extracellular matrix, cartilage
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