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KMID : 1094720060110050396
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Biotechnology and Bioprocess Engineering
2006 Volume.11 No. 5 p.396 ~ p.401
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Antitumor activity of cell suspension culture of green tea seed ( Camellia sinensis L.)
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Choi Jae-Hoon
Yoon Sang-Kun
Lee Keyong-Ho
Seo Min-Soo
Kim Doo-Hwan
Hong Seung-Beom
Kim Ji-Yeon
Paik Hyun-Dong
Kim Chang-Han
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Abstract
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The objective of this study was to investigate the antitumor activity of suspension cultures of tea callus cells grown in the presence of different concentrations of the growth regulator 2,4-dichlorophenoxy acetic acid (2,4-D) with or without light irradiation. The methanol and ethanol extracts of precipitated cells (MEP, EEP) exhibited stronger inhibitory effects on the growth of tumor cell lines than the water extract of precipitated cells (WEP) or the supernatant. Compared to culture under dark conditions, exposure to light irradiation led to significantly higher antitumor activity. The MEP from light irradiated cells at 250 μg/mL with 2.0 mg/L 2,4-D displayed more than 64% growth inhibition of HEP-2 cells, whereas normal cells showed less than 25% growth inhibition. The some fractions of MEP obtained from Diaion HP-20 column chromatography displayed the majority of inhibitory activity against the HEP-2 cell line. These results show that 2,4-D, and light stimulated the synthesis of antitumor compounds.
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KEYWORD
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Camellia sinensis L., green tea seed, cell suspension culture, secondary metabolite, antitumor activity, MTT assay
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