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KMID : 1094720060110050402
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Biotechnology and Bioprocess Engineering
2006 Volume.11 No. 5 p.402 ~ p.407
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Sildenafil citrate induces migration of mouse aortic endothelial cells and proteinase secretion
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Kim Young-Il
Oh In-Suk
Park Seung-Moon
Kim Hwan-Gyu
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Abstract
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Vascular endothelial cells release proteinases that degrade the extracellular matrix (ECM), thus enabling cell migration during angiogenesis and vasculogenesis. Sildenafil citrate stimulates the nitric oxide-cyclic guanosine monophosphate pathway through inhibition of phosphodiesterase type V (PDE5). In this report, we examined the mechanisms underlying sildenafil citrate-induced cell migration using cultured mouse aortic endothelial cells (MAECs). Sildenafil citrate induced migration and proteinase secretion by murine endothelial cells. Sildenafil citrate induced the secretion of matrix metalloproteinase-2 (MMP-2) and MMP-9, which is inhibited by NF-κB inhibitors. Sildenafil citrate also induced the secretion of plasmin, which is inhibited by Pl 3′-kinase inhibitors. It is suggested that sildenafil citrate-induced migrating activity in endothelial cells may be accomplished by increased secretion of proteinases.
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KEYWORD
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sildenafil citrate, migration, MMPs, plasmin
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