|
KMID : 1094720060110050462
|
|
Biotechnology and Bioprocess Engineering
2006 Volume.11 No. 5 p.462 ~ p.465
|
|
|
Removal of aqueous pentachlorophenol by horseradish peroxidase in the presence of surfactants
|
|
Kim E. Y.
Choi Y. J.
Chae H. J.
Chu K. H.
|
|
|
Abstract
|
|
|
|
An important issue in the oxidation of pentachlorophenol (PCP) by the enzyme horseradish peroxidase (HRP) is enzyme inactivation during the reaction. This study was initiated to investigate the ability of two nonionic surfactants (Tween 20 and Tween 80) to mitigate HRP inactivation. The surfactants were tested at concentrations below and above their critical micelle concentrations (CMCs). Enhancement of PCP oxidation was observed at sub-CMCs, indicating effective protection of HRP by the two surfactants. Maximum levels of PCP removal were observed when the concentrations of Tween 20 and Tween 80 were 40 and 50% of the CMCs, respectively. At supra-CMCs, both surfactants caused a noticeable reduction in the extent of PCP removal.
|
|
|
KEYWORD
|
|
|
enzymatic catalysis, horseradish peroxidase, pentachlorophenol, surfactant
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|