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KMID : 1094720070120020080
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 2 p.80 ~ p.85
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Screening of a specific monoclonal antibody against and detection of Listeria monocytogenes whole cells using a surface plasmon resonance biosensor
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Joung Hyou-Arm
Shim Won-Bo
Chung Duck-Hwa
Choi Ho-Suk
Ha Sang-Do
Kim Keun-Sung
Lee Kyu-Ho
Kim Cheol-Ho
Kim Kwang-Yup
Kim Min-Gon
Ahn Jun-Hyoung
Chung Bong-Hyun
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Abstract
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In this study, a specific monoclonal antibody againstListeria monocytogenes was screened using an SPR biosensor Monoclonal antibodies were bound to protein L, after which theL. monocytogenes cells were subjected to an affinity assay. Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surface via an amine coupling method and utilized repeatedly by regeneration. The monoclonal antibody, ‘A18’, was selected and employed for the high-sensitivity detection ofL. monocytogenes. Under optimized conditions, 103 cells/ml or 50 cells were detected by the SPR biosensor.
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KEYWORD
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surface plasmon resonance (SPR), protein L, Listeria monocytogenes, antibody
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