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KMID : 1094720070120020180
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 2 p.180 ~ p.183
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Transient expression of the GUS gene in a unicellular marine green alga, Chlorella sp. MACC/C95 , via electroporation
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Wang Chang-Hai
Wang Yi-Yun
Su Qiao
Gao Xiao-Rong
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Abstract
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A transient expression system for a unicellular marine green alga,Chlorella sp.MACC/C95, was developed using a reporter GUS gene coded for by plasmid pBI121. The results demonstrated a high transformation efficiency could be achieved by using electroporation to deliver DNA into intact cells and the CaMV35S promoter to drive the foreign gene expression inChlorella sp.MACC/C95. The use of a carrier DNA coupled with osmosis treatment improved the transformation efficiency, while linearization of the plasmid had minor effects. Investigation of the effects of DNA concentration and growth phases ofChlorella sp.MACC/C95 on transformation efficiency indicated that the highest level of transient expression was observed when 6 μg mL?1 of plasmid DNA and cells 2?6 days old were used.
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KEYWORD
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Chlorellasp., gene transformation, CaMV35S promoter, transient expression, GUS gene
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