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KMID : 1094720070120030271
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 3 p.271 ~ p.275
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Characterization of extracellular polysaccharides from Nostoc flagelliforme cells in liquid suspension culture
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Jia Shi-Ru
Yu Hai-Feng
Lin Yong-Xian
Dai Yu-Jie
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Abstract
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Nostoc flagelliforme cells were studied with regard to the physico-chemical characterization of the extracellular polysaccharides (EPS) secreted in a liquid suspension culture. The hydrolyzed EPS were determined to be composed of four neutral sugars, which were glucose (43.2%), xylose (20.6%), galactose (29.9%), and mannose (6.3%). The glucuronic acid was the only uronic acid identified in the residue. The apparent molecular weight was estimated at 2.79×105. The Fourier transform infrared spectra showed that the EPS evidenced characteristics typical of non-sulfated polysaccharides. The UV spectrum and Bradford reaction indicated that there were no nucleic acids and proteins in them. The thermal analysis showed a decomposition peak at 245°C on the thermogravimetric (TG) curves. The scanning electron microscope (SEM) analysis indicated that the EPS possessed a porous structure. The observed microstructural irregularities indicated that the polysaccharide was a type of amorphous solid. These results showed that the EPS ofN. flagelliforme cells might be ernployed as a substitute for those normally derived from field colonies. The results of this study may prove to be beneficial to the protection of the natural resource represented byN. flagelliforme.
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KEYWORD
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Nostoc flagelliforme, exocellular polysaccharide, purification, physico-chemical characterization, liquid suspension culture
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