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KMID : 1094720070120040340
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 4 p.340 ~ p.347
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Biocalorimetric and respirometric studies on metabolic activity of aerobically grown batch culture of Pseudomonas aeruginosa
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Sivaprakasam Senthilkumar
Mahadevan Surianarayanan
Bhattacharya Madhuchhanda
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Abstract
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Biocalorimetry has proved to be a useful tool for scale up and control of bioreactors. The findings reported here are fundamental data required for scale up and control of a reactor for the treatment of saline tannery wastewater. The study deals with biokinetics of a halo-tolerant bacteriaPseudomonas aeruginosa isolated from tannery saline wastewater (soak liquor). Batch experiments were performed in a biocalorimeter and the isolated strain was grown in a glucose-limited mineral salt medium (MSM) at optimized growth conditions. Tessier model is found to fit well for the growth ofP. aeruginosa in biocalorimeter. Biokinetic constants are evaluated and simulation is done to validate experimental results with theoretical values. Respirogram and heat profiles are seen to follow the biomass growth curve. Oxycalorific coefficient is validated with the theoretical values and those noticed in the published literature. There is a good correlation between experimentally determined heat yields and the theoretical values predicted by elemental and enthalpy balances. The heat yield and biomass yield values indicated the behavior of the isolated organism in a substrate-limited well defined growth media (MSM)
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KEYWORD
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biocalorimetry, Pseudomonas aeruginosa, metabolic activity, Tessier model, oxygen uptake
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