|
KMID : 1094720070120040348
|
|
Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 4 p.348 ~ p.353
|
|
|
Phytate degradation by immobilized Saccharomyces cerevisiae phytase in soybean-curd whey
|
|
In Man-Jin
Kim Kyung-Hee
Oh Nam-Soon
|
|
|
Abstract
|
|
|
|
Saccharomyces cerevisiae CY phytase-producing cells were immobilized in calcium alginate beads and used for the degradation of phylate. The maximum activity and immobilization yield of the immobilized phytase reached 280 mU/g-bead and 43%, respectively. The optimal pH of the immobilized cell phytase was not different from that of the free cells. However, the optimum temperature for the immobilized phytase was 50°C, which was 10°C higher than that of the free cells; pH and thermal stability were enhanced as a consequence of immobilization. Using the immobilized phytase, phytate was degraded in a stirred tank bioreactor. Phytate degradation, both in a buffer solution and in soybean-curd whey mixture, showed very similar trends. At an enzyme dosage of 93.9 mU/g-phytate, half of the phytate was degraded after 1 h of hydrolysis. The operational stability of the immobilized beads was examined with repeated batchwise operations. Based on 50% conversion of the phytate and five times of reuse of the immobilized beads, the specific degradation (g phytate/g dry cell weight) for the immobilized phytase increased 170% compared to that of the free phytase.
|
|
|
KEYWORD
|
|
|
phytate, degradation, immobilized phytase, Saccharomyces cerevisiae
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|