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KMID : 1094720070120050525
Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 5 p.525 ~ p.530
Effect of mobile phase additives on resolution of some nucleic compounds in high performance liquid chromatography
Jin Chun-Hua

Koo Yoon-Mo
Choi Dae-Ki
Row Kyung-Ho
Abstract
Here we investigate the chromatographic behavior, with reversed-phase high performance liquid chromatography (RP-HPLC) of nucleic compounds (nucleobases, nucleosides, and nucleotides) on a C18 column in several different mobile phase additives, including1-butyl-3-methylimidazolium tetrafuloroborate ([BMIm][BF4]), 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]) ionic liquids, ammonium formate, and potassium phosphate. The effect of the alkyl group length, the imidazolium ring, and the ionic liquid¡¯s counterions on retention and resolution of the samples were tested. The results show the potential application of a used buffer system, ion pairing system, and ionic liquid as mobile phase additives in liquid chromatography resolution of nucleic compounds.
KEYWORD
ionic liquid, buffer, ion pairing, RP-HPLC, nucleic compounds
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