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KMID : 1094720070120050525
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 5 p.525 ~ p.530
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Effect of mobile phase additives on resolution of some nucleic compounds in high performance liquid chromatography
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Jin Chun-Hua
Koo Yoon-Mo
Choi Dae-Ki
Row Kyung-Ho
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Abstract
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Here we investigate the chromatographic behavior, with reversed-phase high performance liquid chromatography (RP-HPLC) of nucleic compounds (nucleobases, nucleosides, and nucleotides) on a C18 column in several different mobile phase additives, including1-butyl-3-methylimidazolium tetrafuloroborate ([BMIm][BF4]), 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]) ionic liquids, ammonium formate, and potassium phosphate. The effect of the alkyl group length, the imidazolium ring, and the ionic liquid’s counterions on retention and resolution of the samples were tested. The results show the potential application of a used buffer system, ion pairing system, and ionic liquid as mobile phase additives in liquid chromatography resolution of nucleic compounds.
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KEYWORD
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ionic liquid, buffer, ion pairing, RP-HPLC, nucleic compounds
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