|
KMID : 1094720070120060594
|
|
Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 6 p.594 ~ p.600
|
|
|
Increased hGM-CSF production and secretion with Pluronic F-68 in transgenic Nicotiana tabacum suspension cell cultures
|
|
Cho Jong-Moon
Kwon Jun-Young
Lim Jung-Ae
Kim Dong-Il
|
|
|
Abstract
|
|
|
|
The effects of Pluronic F-68, a nonionic surfactant, on the production and secretion of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) in a transgenicNicotiana tabacum cell suspension culture were investigated in this study. The addition of Pluronic F-68 was shown to extend cell survival in the stationary phase, but had no influence on effective initial cell growth. With regard to production, it increased the level of extracellular hGM-CSF two-fold. This may be attributable not only to the enhanced expression level, but also to the improved permeability of the cell membrane due to the interaction between Pluronic F-68 and the cell membrane and cell wall. The effect of Pluronic F-68 on the production and secretion of hGM-CSF in a bioreactor was also evaluated. hGM-CSF production in the bioreactor after the addition of Pluronic F-68 proved more effective than in flask cultures.
|
|
|
KEYWORD
|
|
|
transgenic plant cell culture, hGM-CSF, Pluronic F-68, secretion, permeabilization
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|