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KMID : 1094720070120060696
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 6 p.696 ~ p.706
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Enhanced In vitro chondrogenic differentiation of murine embryonic stem cells
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Hwang Yu-Shik
Bishop Anne E.
Polak Julia M.
Athanasios Mantalaris
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Abstract
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Current approaches have focused on deriving ESCs differentiation into chondrocytes from a cell source of spontaneously formed intact mesoderm in EB formation, resulting in limited yield. Our study aimed at upregulating chondrogenic differentiation of murine ESCs by enhancing mesoderm formation. Specifically, culture of mESCs with conditioned medium from a human hepatocarcinoma cell line resulted in a cell population with a gene expression pattern similar to that of primitive streak/nascent mesoderm, including up-regulation of brachyury, goosecoid, nodal, and cripto. From this cell population, reducing the embryoid body formation time resulted in enhancement of chondrogenic differentiation as evidenced by larger Alcian blue-stained cartilage nodules, higher production of sulfated glycosaminoglycan matrix, the presence of well-organised type II collagen and type II collagen, aggrecan and sox-9 gene expression. In conclusion, we present here a new approach to the generation of chondrocytes from mESCs that enhances yields and, thus, could have widespread applications in cartilage tissue engineering.
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KEYWORD
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murine embryonic stem cells - HepG2-conditioned medium, primitive streak/nascent mesoderm, embryoid body, chondrogenic differentiation, cartilage nodule
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