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KMID : 1094720080130030274
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 3 p.274 ~ p.278
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Development and optimization of fractional precipitation for the pre-purification of (+)-dihydromyricetin
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Lee Kang-Hee
Kim Jin-Hyun
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Abstract
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A novel fractional precipitation process, both simple and efficient, was developed for producing (+)-dihydromyricetin in high purity and high yield from crude extracts. The optimal acetone composition in water, initial (+)-dihydromyricetin concentration in crude extract, storage temperature, storage time, and pH were 1/5 (v/v), 0.1 g/mL, 4°C, 32 h, and 9.0, respectively. Crude extracts were efficiently pre-purified using fractional precipitation of (+)-dihydromyricetin by differences of solubility in an acetone solution, increasing purity from 55.0 to over 84.9% with an overall yield of 97.5%. The use of fractional precipitation for pre-purification allowed for rapid and efficient separation of (+)-dihydromyricetin from interfering compounds and dramatically increased the purity of crude (+)-dihydromyricetin for subsequent purification steps.
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KEYWORD
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(+)-dihydromyricetin, fractional precipitation, pre-purification, optimization
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