KMID : 1094720080130050566
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Biotechnology and Bioprocess Engineering 2008 Volume.13 No. 5 p.566 ~ p.570
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Effect of crab shell size on bio-demineralization with lactic acid-producing bacterium, Lactobacillus paracasei subsp. tolerans KCTC-3074
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Oh Kyung-Taek
Kim Young-Ju Nguyen Van-Nam Jung Woo-Jin Park Ro-Dong
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Abstract
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Demineralization (DM) from crab shell (CS) waste was carried out using a lactic acid-producing bacterium, Lactobacillus paracasei subsp. tolerans KCTC-3074 for 7 days at 25, 30, and 35¡ÆC. DM rates were 89¡92% and slightly affected by temperature. DM was also performed for four particle-sized shell samples (0.84¡3.35, 3.35¡10, 10¡20, and 20¡35 mm) with 10% inoculum, 5% shell, and 10% glucose at 30¡ÆC and 180 rpm for 7 days. It was found out that the shell size had a slight effect on the rate of DM. Negative relationships were found between DM and residual dry weight (r2 = 0.960), and between DM and pH (r2 = 0.906). Conversely, positive relationships were found between DM and medium protein (r2 = 0.696), and between DM and total titratable acidity (r2 = 0.630).
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KEYWORD
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bio-demineralization, crab shell waste, Lactobacillus paracasei subsp. tolerans KCTC-3074, total titratable acidity
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