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KMID : 1094720080130060683
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 6 p.683 ~ p.689
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Improved DNA chip with poly(amidoamine) dendrimer peripherally modified with biotin and avidin
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Lim Sung-Bin
Kim Kyung-Woo
Lee Chang-Woo
Kim Hyo-Sun
Lee Chang-Soo
Oh Min-Kyu
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Abstract
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Important properties for a biosensor are the sensitive detection of target DNA at low concentration, the specific and accurate distinction of the target and other DNA having a similar sequence, and measurement capability over a wide range of target concentrations. To these ends, generation 3 polyamidoamine (PAMAM) dendrimer was used to improve DNA chip properties. PAMAM dendrimer surface amine moieties were modified to biotin and immobilized on glass slides using biotin-avidin conjugation. The surface morphologies of the avidin-biotin-dendrimer complexes were observed using atomic force microscopy and scanning electron microscopy. Detection sensitivity for fluorescence-labeled target DNA increased approximately 4-fold by the dendrimer coating. Dendrimer coating also markedly improved the dynamic range and detection of single nucleotide polymorphisms. Dendrimer complex morphology had little effect on the sensitivity.
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KEYWORD
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PAMAM dendrimer, DNA chip, fluorescence, avidin-biotin conjugation
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