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KMID : 1094720080130060690
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 6 p.690 ~ p.696
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Effect of cultivation conditions on trehalose content and viability of brewing yeast following preservation via filter paper or lyophilization methods
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Cheong Chul
Wackerbauer Karl
Beckmann Martin
Jang Ki-Hyo
Kang Soon-Ah
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Abstract
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The effects of variations in cultivation conditions on trehalose concentration and the viability of brewing yeasts following preservation by filter paper or lyophilization methods were evaluated. In case of filter paper preservation, the cultivation period had no affect on yeast viability, while agitation and aeration during cultivation had a positive effect regarding viability of the bottom-fermenting strains, Rh and Frank. For effective preservation, it was necessary to harvest yeast cells from the stationary phase during cultivation. For lyophilization preservation, the yeast strains tested showed a negative effect on viability, independent of strain or cultivation method. No significant correlation was found between trehalose concentration and yeast viability following either filter paper or lyophilization preservation. However, the filter paper preservation method was suitable for both bottom and top brewing yeast strains with regard to feasibility, viability, and maintenance of the yeast’s specific character.
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KEYWORD
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filter paper preservation, trehalose, viability, brewing yeast
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