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KMID : 1094720080130060752
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 6 p.752 ~ p.757
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Functional characterization of orf6 and orf9 genes involved in the biosynthesis of L -oleandrose from Streptomyces antibioticus Tu99
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Pageni Binod Babu
Oh Tae-Jin
Yoo Jin-Cheol
Sohng Jae-Kyung
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Abstract
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Two genes, orf6 and orf9 located in the L-oleandrose sugar biosynthetic gene cluster of Streptomyces antibioticus Tu99. NovU has been characterized as C-5 methyltrnaferase involved in noviose biosynthetic pathway. We have cloned and heterologously expressed the orf6, orf9, and novU genes in S. venezuelae YJ003-OTBP1. This established the function of orf6 and orf9 as 4-ketoreductase and 3-epimerase, respectively. All of analytical data of the noviosylated 10-deoxymethynolide also is in support of proving their functions. Furthermore biosynthetic pathway 5,5-gem-dimethyl-6-deoxyglucose (TDP-Lnoviose) has been proposed.
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KEYWORD
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deoxysugar biosynthesis, 3-epimerase, 4-ketoreductase, L-noviose, Streptomyces antibioticus Tu99
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