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KMID : 1094720090140020134
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 2 p.134 ~ p.139
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Enhancement of α-ketoglutarate Production in Torulopsis glabrata: Redistribution of Carbon Flux from Pyruvate to α-ketoglutarate
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Zhang Dandan
Liang Nan
Zhongping Shi
Chen Jian
Guocheng Du
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Abstract
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This manuscript aimed at increasing the production of α-ketoglutarate by the multi-vitamin auxotrophic yeast Torulopsis glabrata CCTCC M202019. The carbon flux was redistributed from pyruvate to α-ketoglutarate node by manipulating the specific activity of pyruvate dehydrogenase complex (PDH), pyruvate carboxylase (PC), pyruvate decarboxylase (PDC), and α-ketoglutarate dehydrogenase complex (KGDH). By proper increase of PDH, PC, and PDC activities, α-ketoglutarate in fermentation broth could be accumulated to the levels of 17.1 g/L, 21.6 g/L, and 31.2 g/L, respectively. In addition, decrease in the specific activity of KGDH also resulted in an enhanced α-ketoglutarate synthesis. With a proposed combinational enzymes regulation strategy, the highest α-ketoglutarate concentration of 37.7 g/L was achieved.
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KEYWORD
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Torulopsis glabrata, α-ketoglutarate, pyruvate
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