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KMID : 1094720090140020220
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 2 p.220 ~ p.224
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Optimization of Process Variables in Castor Oil Hydrolysis by Candida Rugosa Lipase with Buffer as Dispersion Medium
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Debajyoti Goswami
Jayanta Kumar Basu
Sirshendu De
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Abstract
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In this study, castor oil is hydrolyzed in presence of Candida rugosa lipase, while in the buffer (aqueous) phase as a dispersion medium. The following conditions were used to optimize the process: speed of agitation, initial pH of buffer phase, temperature, and ratio of buffer phase volume to oil weight. The optimal conditions are 1,100 rpm, pH 6.5, temperature 35°C, and 3:1 buffer phase volume to oil weight ratio. Under these described conditions, the reusability of lipase was tested and it was found that nearly 80% of original hydrolysis percentage was achieved after the first recycle.
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KEYWORD
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castor oil, candida rugosa lipase, hydrolysis, ricinoleic acid
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