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KMID : 1094720090140030322
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 3 p.322 ~ p.329
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Influence of Sodium Orthovanadate on the Production of Astaxanthin from Green Algae Haematococcus lacustris
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Tran Ngoc-Phuong
Park Jae-Kweon
Kim Z-Hun
Lee Choul-Gyun
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Abstract
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Astaxanthin production is commonly induced under stress conditions such as nutrient deficiency (N or P), high light stress, and variations of temperature, high NaCl concentrations, and other factors. The objective of the present study is the analysis of the effect of oxidative stress by sodium orthovanadate (SOV), a nonspecific inhibitor of protein tyrosine phosphatases, on the cells growth and astaxanthin production of H. lacustris. In the presence of SOV (lower than 5.0 mM), maximum growth of H. lacustris obtained was 2.4×105 cells/mL in MBBM medium at 24°C under continuous illumination (40 μE/m2/s) of white fluorescent light, with continuous aeration of CO2 (0.2 vvm). Total carotenoids accumulated per cell biomass unit treated with 2.5 mM SOV has approximately shown 2.5 folds higher than the control after short period of SOV induction time as 2 days, despite that cells were grown under normal light. Meanwhile, maximal astaxanthin production from H. lacustris was 10.7 mg/g biomass in MBBM with 5 days of continuous illumination at 40 μE/m2/s, which has been established as optimal light intensity for the control culture of H. lacustris. Treating algae H. lacustris with sodium orthovanadate showed promoting the accumulation of astaxanthin by advancing either the inhibition of dephosphorylation or synthesis of ATP. Its potential role of PTPases in microalgae H. lacustris is discussed.
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KEYWORD
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Haematococcus lacustris, astaxanthin, sodium orthovanadate, protein tyrosine phosphatase, photobioreactor
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