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KMID : 1094720090140040436
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 4 p.436 ~ p.442
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Isolation and Biochemical Characterization of Collagens from Seaweed Pipefish, Syngnathus Schlegeli
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Khan Sher Bahadar
Qian Zhong-Ji
Ryu Bo-Mi
Kim Se-Kwon
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Abstract
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Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from the seaweed pipefish (Syngnathus schlegeli) and partially characterized. The amount of collagens isolated in the subsequent treatments was 5.5% of ASC and 33.2% PSC on the basis of lyophilized pipefish body weight, respectively. According to the electrophoretic pattern and CM-cellulose column chromatogram, the collagens might be classified as type I collagens, containing α1 and α2 chain. The imino acid content of collagen from pipefish was lower than those of mammalian collagens as also were the denaturation temperatures (Td) of collagens were 34.8°C and 35.1°C, respectively. This study shows that there is a possibility to use pipefish collagen as the alternative source of collagen from industrial purposes and subsequently it may evaluate the economical value of the seaweed pipefish.
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KEYWORD
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collagen, seaweed pipefish, isolation, characterization
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