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KMID : 1094720090140040503
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 4 p.503 ~ p.512
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Excellent Laundry Detergent Compatibility and High Dehairing Ability of the Bacillus pumilus CBS Alkaline Proteinase (SAPB)
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Bassem Jaouadi
Semia Ellouz-Chaabouni
Mamdouh Ben Ali
Ezzedine Ben Messaoud
Belgacem Naili
Abdelhafidh Dhouib
Samir Bejar
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Abstract
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The newly Tunisian soil-isolated bacterium, producing the alkaline proteinase termed SAPB that was already purified and characterized [1], was assigned as Bacillus pumilus CBS strain on the basis of biochemical properties and 16S rRNA gene sequencing. The maximum protease activity recorded after 24 h of incubation in an optimized medium at 37°C was 6,500 U/mL in shaking flask culture and 25,000 U/mL in fermentor. SAPB showed excellent stability and compatibility in laundry detergent retaining more than 98% of its initial activity after pre-incubation for 1 h at 40?C with Det, followed by OMO (97%), Dinol (94%), and Dixan (93%). Examination of various stained cloth pieces exhibited a remarkable efficiency in the removal of blood and chocolate stains. More interestingly, SAPB demonstrated powerful dehairing capabilities of hair removal from skin with minimal damage on the collagen and a nearly complete feather-degradation. Likewise, Bacillus pumilus CBS effectively degraded feather-meal (98.5%), chicken feather (92%), goat hair (80%), and bovine hair (68%) whereas sheep wool under went less degradation. Keratin-degradation resulted in sulfhdryl group formation (0.95~3.91 μM).
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KEYWORD
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Alkaline proteinase, Bacillus pumilus CBS, laundry detergent, dehairing function, keratin-degradation
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