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KMID : 1094720090140040536
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 4 p.536 ~ p.542
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Removal of α-naphthol and Other Phenolic Compounds from Polluted Water by White Radish (Raphanus sativus) Peroxidase in the Presence of an Additive, Polyethylene Glycol
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Ashraf Humaira
Husain Qayyum
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Abstract
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Role of white radish peroxidase has been investigated in the treatment of water contaminated with phenols, particularly α-naphthol. Water polluted with α-naphthol was treated with white radish peroxidase under various experimental conditions. The treatment of α-naphthol polluted water by this enzyme in presence of polyethylene glycol enhanced its removal. Studies carried out in absence of polyethylene glycol showed only 36% of α-naphthol removal however, 96% of it was removed in presence of 0.1 mg/mL of polyethylene glycol in 100 mM sodium phosphate buffer, pH 6.5, and 0.75 mM H2O2 at 40°C. The other phenols oxidized and removed from waste water under similar experimental conditions were 18%, m-cresol; 30%, p-chlorophenol; 62%, p-bromophenol; 20%, benzyl alcohol; 21%, quinol; 38%, 2,6-dichlorophenol; 13%, 2,4-dichlorophenol; and 2%, native phenol. Mixtures of different phenolic compounds removed under identical treatment conditions were 63%, A; 40%, B; 52%, C; 41%, D; 72%, E; 66%, F; and 72%, G. Thus, peroxidase in presence of an additive, polyethylene glycol could be a suitable tool for the removal of phenolic compounds from industrial effluents.
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KEYWORD
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peroxidase, wastewater, phenols, polyethylene glycol
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