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KMID : 1094720090140050559
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 5 p.559 ~ p.564
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Production of β-carotene by Recombinant Escherichia coli with Engineered Whole Mevalonate Pathway in Batch and Fed-batch Cultures
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Kim Jung-Hun
Kim Seon-Won
Do Quynh Anh Nguyen
Li He
Kim Sung-Bae
Seo Yang-Gon
Yang Jae-Kyung
Chung In-Young
Kim Dae-Hwan
Kim Chang-Joon
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Abstract
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Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for β-carotene biosynthesis, was utilized for production of β-carotene in bioreactor cultures. Optimum culture conditions were established in batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific growth rate and volumetric productivity in batch cultures at 37oC were 1.7-fold and 2-fold higher, respectively, than those at 28oC. Glycerol was superior to glucose as a carbon source. Maximum β-carotene production (titer of 663 mg/L and overall volumetric productivity of 24.6 mg/L × h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast extract in pH-stat fed-batch culture.
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KEYWORD
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recombinant Escherichia coli, engineered whole mevalonate pathway, β-carotene, bioreactor culture, fed-batch culture
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