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KMID : 1094720090140050619
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 5 p.619 ~ p.624
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Accumulation of Sesquiterpenes and Polysaccharides in Cells of Zedoary (Curcuma zedoaria Roscoe) Cultured in a 10 L Bioreactor
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Nguyen Hoang Loc
Vo Chau Tuan
Doan Huu Nhat Binh
Truong Thi Bich Phuong
Kim Tae-Geum
Yang Moon-Sik
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Abstract
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We developed a cell suspension culture system for zedoary (Curcuma zedoaria Roscoe), using 100 g fresh weight inoculum in a batch culture. The maximum cell biomass of 68.46 g/L fresh weight was obtained after 14 days of culture in a 10 L bioreactor with a pitch-blade impeller maintained at an agitation speed of 150 rpm and an aeration rate of 2.5 L/min. The accumulation of sesquiterpenes and polysaccharide in zedoary cells from 2 to 18 days was measured by HPLC and a phenol-sulfuric acid assay, respectively. The total polysaccharide concentration increased between 2 to 10 days of culture and reached a maximum value of 6.55%. HPLC revealed several eluted peaks of sesquiterpenes, which increased in amplitude from days 2 to 10. Furthermore, our results indicated that biotransformation occurred in the cell suspension, transforming certain sesquiterpenes into other types during culture.
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KEYWORD
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Cell suspension, Curcuma zedoaria, HPLC, polysaccharide, sesquiterpenes
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