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KMID : 1094720090140050675
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 5 p.675 ~ p.679
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Simultaneous Quantification of Multiple Alkaloids in Sophora Flavescens Ait and Human Urine by HPLC
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Liu Jun-Yu
Yan Hong-Yuan
Row Kyung-Ho
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Abstract
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This study developed a simple and sensitive liquid chromatography method to quantify the multiple active alkaloids (matrine, sophocarpine, and sophoridine) from sophora flavescens ait in urine samples simultaneously. The results showed that liquid-liquid extraction using water as the solvent could effectively isolate all the analyses with the lowest matrix effects. A low concentration of diethylamine (0.07%, v/v) in the mobile phase improved dramatically the resolution of all the alkaloids with less interference from the matrix. Good linearity ranging from 0.025 to 1250.0 μg/mL for all analytes was obtained, and the accuracy and precision varied from 73.25 to 87.86% and 1.4 to 2.2%, respectively. Finally, the suitability of the proposed method was demonstrated in the urinary drug excretion of 4 volunteers receiving an oral dose of a sophora flavescens ait extract. The intra and inter RSD were ≤ 1.9%.
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KEYWORD
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matrine, sophocarpine, sophoridine, HPLC, urine, sophora flavescens ait
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