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KMID : 1094720090140060828
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 6 p.828 ~ p.834
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Pretreatment and Saccharification of Rice Hulls for the Production of Fermentable Sugars
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Wei Gong-Yuan
Gao Wa
Jin II-Hyuck
Chung Chung-Han
Lee Jin-Woo
Yoo Su-Young
Lee Jai-Heon
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Abstract
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Hydrolytic conditions of rice hulls by acid and alkaline treatments before enzymatic saccharification were optimized in this study. Based on the results of single-factor experiments and an orthogonal array experiment, reaction time was found to be the most important factor for the acidic hydrolysis of rice hulls. Maximum yield of sugars from 1 g of rice hulls by acidic treatment under optimized conditions was 213.6 mg. The yield of lignin removal from acidic pretreated rice hulls by alkaline treatment increased with increase in reaction temperature and time. The amount of sugars obtained from 1 g of pretreated rice hulls by enzymatic saccharification was 307.7 mg, and the conversion rate of sugars from crude fibers in pretreated rice hulls was about 72%. Instrumental analyses with FTIR and SEM indicated that lignin in rice hulls was partially removed by alkaline treatment, and the structure of rice hulls became deformed and more fibers were exposed to cellulases after acidic treatment.
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KEYWORD
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rice hulls, pretreatment, hydrolysis, saccharification, cellulase, fermentable sugars
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