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KMID : 1094720090140060842
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 6 p.842 ~ p.847
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Fabrication and Characterization of Silver Nanoparticle and its Potential Antibacterial Activity
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P. Prema
Rincy Raju
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Abstract
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In the present research silver nanoparticle was fabricated by chemical reduction of silver salt (Silver nitrate, AgNO3) solution. Sodium citrate was used as a reducer. The formation of silver nanoparticle was observed visually by color change (greenish yellow). The surface plasmon resonance peak in absorption spectra of silver nanoparticle showed an absorption maximum at 420 nm in UV-VIS spectrometry. The X-ray diffraction pattern showed the presence of sharp reflections at 111, 200, 220, and 311. This would indicate the presence of silver nanoparticle. The scanning electron micrograph revealed that the average size of silver nanoparticle was 21.22 ?5.17 nm. Silver nanoparticle exhibited better antimicrobial activity against Staphylococcus aureus than the other bacterial pathogens. The correlation coefficient between silver nanoparticles and selected bacterial pathogens revealed that there is a strong negative correlation with Escherichia coli, S. aureus and Klebsiella pneumonia (r = ?0.975, ?0.993, and ?0.998, respectively).
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KEYWORD
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silver nanoparticle, UV, XRD, SEM, antibacterial activity
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