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KMID : 1094720100150050837
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 5 p.837 ~ p.845
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Optimization of mineral salts in medium for enhanced production of pullulan by Aureobasidium pullulans HP-2001 using an orthogonal array method
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Gao Wa
Kim Yi-Joon
Chung Chung-Han
Li Jianhong
Lee Jin-Woo
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Abstract
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Based on intuitive analyses and statistical calculations using data from orthogonal array experiments, the optimal concentrations of K2HPO4, NaCl, MgSO4·7H2O, and (NH4)2SO4 in cell growth medium of Aureobasidium pullulans HP-2001 were measured as 7.5, 1.0, 0.1, and 2.4 g/L, respectively, whereas those for the production of pullulan were 2.5, 0.25, 0.8, and 0.3 g/L, respectively. The most important factor for cell growth and production of pullulan by A. pullulans HP-2001 was identified as K2HPO4. Optimal concentrations of glucose and yeast extract, along with the initial pH of the cell growth medium of A. pullulans HP-2001 containing optimized salt concentrations, were found to be 100.0, 10.0, and 6.0 g/L, respectively, whereas those for the production of pullulan were 100.0, 2.5, and 6.0 g/L, respectively. Conversion rates of pullulan from 10.0, 25.0, 50.0, 75.0, and 100.0 g/L of glucose in the presence of optimized salt concentrations were 26.0, 25.2, 22.4, 17.9, and 14.1%, respectively, whereas those in the presence of previously reported salt concentrations were 26.6, 25.2, 19.9, 14.3, and 11.7%, respectively. Optimal salt concentrations for the production of pullulan by A. pullulans HP-2001 varied according to the concentrations of the carbon and nitrogen sources, especially at higher concentrations.
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KEYWORD
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pullulan, aureobasidium pullulans, optimization of salts, optimization of medium, orthogonal array method
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