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KMID : 1094720120170010168
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Biotechnology and Bioprocess Engineering
2012 Volume.17 No. 1 p.168 ~ p.175
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An aqueous extract of Zingiber officinale Roscoe protects mouse primary hepatic cells against hydrogen peroxide-induced oxidative stress
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Oh Su-Young
Lee Ji-Hye
Lee Ji-Seon
Seo Sang-Hee
Ma Jin-Yeul
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Abstract
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Hepatocytes exposed to an oxidative stressor such as hydrogen peroxide (H2O2) are potentially sensitized to cell death; thus, reactive oxygen species (ROS) are considered to be critical mediators of liver damage. Zingiber officinale Roscoe (ZO), also known as ginger, is cultivated commercially in China, India, Korea, and other parts of the world. In addition, it is used as a spice and flavoring agent and is also purported to possess a number of medicinal properties. In the present study, we examined the protective effect of ZO against cell damage caused by H2O2-induced oxidative stress. ZO reduced H2O2-induced apoptotic signals and the levels of intracellular ROS. ZO pretreatment also increased the phosphorylation of c-Jun, and JNK kinase. The expression of heme oxygenase-1 (HO-1) and heat shock protein 72 (HSP72) were increased by ZO pretreatment more than H2O2 treatment. In addition, siRNA-mediated knockdown of HO-1 and HSP72 decreased protective effect of ZO pretreatment. Our data suggest that ZO decreases ROS levels and the expressions of HO-1 and HSP72 are involved in the hepatocyte protective function of ZO against H2O2.
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KEYWORD
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liver, Zingiber officinale Roscoe, ROS, apoptosis, HO-1, HSP72
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