KMID : 1094720120170020393
|
|
Biotechnology and Bioprocess Engineering 2012 Volume.17 No. 2 p.393 ~ p.397
|
|
Regioselective enzymatic procedure for preparing 3¡Ç-O-stearoyl-6-azauridine by using Burkholderia cepacia lipase
|
|
Wang Zhao-Yu
Bi Yan-Hong Zong Min-Hua
|
|
Abstract
|
|
|
Previous studies on the lipase-mediated acylation of 6-azauridine with vinyl stearate in organic solvents revealed that while preparing a potential prodrug, 3¡Ç-O-stearoyl-6-azauridine, a lipase from Burkholderia cepacia showed high regioselectivity toward the second hydroxyl group. The most suitable reaction solvent, molar ratio of vinyl stearate to 6-azauridine, and reaction temperature were anhydrous acetone, 15:1, and 45¡ÆC, respectively. Under these conditions, the initial reaction rate, 3¡Ç-regioselectivity, and maximum substrate conversion were as high as 10.4 mM/h, 86.0, and 99.0%, respectively.
|
|
KEYWORD
|
|
6-azauridine, Burkholderia cepacia lipase, enzyme catalysis, organic solvent, regioselective acylation
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|