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KMID : 1094720120170051076
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Biotechnology and Bioprocess Engineering
2012 Volume.17 No. 5 p.1076 ~ p.1079
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Genotyping of the porcine ryanodine receptor 1 (RYR1) and estrogen receptor 1 (ESR1) genes by high resolution melting (HRM) approach
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Ma Jideng
Li Mingzhou
Wang Huiyu
Li Xuewei
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Abstract
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During the last decade, DNA mutations in the porcine ryanodine receptor 1 gene (RYR1, C1843T) and the estrogen receptor 1 gene (ESR1, T1665G), have been widely used in marker-assisted selection (MAS) for the pig industry. These 2 well-characterized SNPs in RYR1 and ESR1 are responsible for porcine stress syndrome (PSS) and litter size, respectively. Here, we describe a reliable, high-efficiency method for the genotyping of these 2 genes using the high-resolution melting (HRM) method. The HRM approach exhibited high-accuracy and repeatability, comparable with the classic PCR-restriction fragment length polymorphism (PCR-RFLP) approach, and is potentially suitable for large-scale genotyping in commercial pig farms.
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KEYWORD
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estrogen receptor 1 gene, genotyping, highresolution melting, pig, ryanodine receptor 1 gene
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