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KMID : 1094720140190060973
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Biotechnology and Bioprocess Engineering
2014 Volume.19 No. 6 p.973 ~ p.977
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Pikromycin production stimulation through antibiotic down-regulatory gene disruption in Streptomyces venezuelae
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Woo Min-Woo
Nah Hee-Ju
Choi Si-Sun
Kim Eung-Soo
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Abstract
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The wblA gene, which encodes a whiB-like putative transcription factor, has been widely reported as an antibiotic biosynthesis down-regulator in Streptomyces coelicolor. The wblA ortholog from Streptomyces venezuelae was identified by sequence alignment with wblA from S. coelicolor. To determine the biological significance of wblA from S. venezuelae, chromosomal disruption of the wblA sve gene was performed by homologous recombination using the pKC1139 vector. The phenotypic difference between S. venezuelae and S. venezuelaeΔwblA sve was shown, and pikromycin production was quantified by HPLC analysis. Production of pikromycin by S. venezuelaeΔwblA sve was approximately 3.5-fold higher compared to that by S. venezuelae. To further show that the wblA sve gene was implicated in pikromycin production, wblA sve was cloned into Streptomyces integrative expression vector, which was independently introduced into both S. venezuelae and S. venezuelaeΔwblA sve by conjugation. HPLC analysis of complementation and overexpression strains showed an approximately 2.5-fold reduction in pikromycin production by the wblA sve overexpression mutant.
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KEYWORD
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wblA, Pikromycin, Streptomyces venezuelae
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