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KMID : 1094720150200040708
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 4 p.708 ~ p.713
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Structural characterization and temperature-dependent production of C17-fengycin B derived from Bacillus amyloliquefaciens subsp. plantarum BC32-1
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Nam Ji-Young
Jung Min-Young
Kim Pyoung-Il
Lee Hyang-Burm
Kim Si-Wouk
Lee Chul-Won
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Abstract
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Cyclic lipopeptides were produced by Bacillus amyloliquefaciens subsp. plantarum strain BC32-1 that was isolated from yellow loess soil in the Jeonnam province of South Korea. Several lipopeptides were isolated from the bacteria using organic solvent extraction and reverse-phase high-performance liquid chromatography (RP-HPLC). Purified iturin-, surfactin-, and fengycin-type lipopeptides were identified using liquid chromatographymass spectrometry (LC-MS) analysis. Among the lipopeptides, C17-fengycin B showed strong antifungal activity against the phytopathogenic fungus, Fusarium oxysporum f. sp. radicis-lycopersici, and then the fengycin was further characterized by UV, Fourier transform-infrared spectroscopy (FT-IR), and LC-MS/MS analyses. C17-fengycin B was highly produced at quantities of up to 15 μg/mL at 37°C, whereas little amount of the fengycin was produced at 25°C. Purified C17-fengycin B inhibited mycelial growth of F. oxysporum with a minimal inhibitory concentration of 50 μg/mL. This study suggests that C17-fengycin B is a major antifungal component produced by the BC32-1 strain that could be used as an environmentally friendly agent to control the phytopathogenic F. oxysporum.
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KEYWORD
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antifungal lipopeptide, Bacillus amyloliquefaciens subsp. plantarum, biocontrol, biosurfactant, fengycin
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