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KMID : 1094720150200050956
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 5 p.956 ~ p.964
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Multiplexed hydrogel microparticle suspension arrays for facile ribosomal RNA integrity assays
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Duenas Yader
Lee Jae-Hun
Jung Suk-Won
Yi Hyun-Min
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Abstract
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Reliable RNA integrity assay is important for a wide range of applications in genomics and diagnostics, yet the existing technologies have certain limitations such as large amount of sample required, high cost of equipment and/or long turnaround times. We report a simple assay method to analyze bacterial ribosomal RNA (rRNA) from complex total RNA samples utilizing shape-encoded and single-stranded DNA-conjugated hydrogel microparticle suspension arrays with no need for target amplification and under standard fluorescence imaging conditions. We show that our simple microparticle-based sensing scheme is reliable, sequence-specific and presents a responsive binding behavior to target RNA concentrations. Moreover, the relative stability of 16S and 23S rRNA can be assessed in a simple shape encoding-based multiplexed format. Combined, these findings represent a significant step toward cheap, fast, simple, and reliable assays for the analysis of rRNA and general RNA integrity.
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KEYWORD
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hydrogel microparticles, rRNA assay, shape-encoded multiplexing
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