KMID : 1094720160210050627
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Biotechnology and Bioprocess Engineering 2016 Volume.21 No. 5 p.627 ~ p.633
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Selective determination of dopamine with an amperometric biosensor using electrochemically pretreated and activated carbon/tyrosinase/Nafion¢ç-modified glassy carbon electrode
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Rahman Siti Fauziyah
Min Kyoung-Seon Park Seok-Hwan Park Jae-Hee Yoo Jin-Cheol Park Don-Hee
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Abstract
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Dopamine, the most important neurotransmitter in the human brain, controls various functions. Dopamine deficiency causes fatal neurological disorders such as Parkinson¡¯s disease. Even though various types of electrochemical sensors have been studied to measure dopamine levels, they often have poor selectivity for dopamine due to co-existence of interfering substances (e.g. ascorbic acid). Herein, we aimed to develop a highly sensitive dopamine detection method in the co-existence of ascorbic acid, a major interfering substance in real sample by designing an electrochemically pretreated and activated carbon/tyrosinase/Nafion¢ç-modified GCE as an amperometric dopamine biosensor. To maximize the biosensor performance, pH, volume of Nafion¢ç, and scan rate were optimized. This electrochemically pretreated and activated carbon/tyrosinase/ Nafion¢ç-modified GCE could detect as low as 50 ¥ìM of dopamine with a wide linear range (50 ~ 1,000 ¥ìM) within a few seconds. In addition, it had a sensitivity of 103mAM/cm2, which was higher than all previously reported tyrosinasebased dopamine biosensors. In addition, interference effect caused by 4 mM of ascorbic acid was negligible in the co-existence of 1 mM of dopamine. Consequently, this electrochemically pretreated and activated carbon/tyrosinase/ Nafion¢ç-modified GCE might be applicable as amperometric biosensor for selective detection of dopamine in real samples with interfering substances.
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KEYWORD
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dopamine, selectivity, biosensors, tyrosinase
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