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KMID : 1094720170220020161
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Biotechnology and Bioprocess Engineering
2017 Volume.22 No. 2 p.161 ~ p.169
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A noninvasive online system for biomass monitoring in shaker flasks using backward scattered light
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Mao Jian
Yan Yingge
Eichstadt Olaf
Chen Xianshuai
Wang Zuowei
Cui Jinming
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Abstract
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This paper presents a noninvasive optical sensor system for monitoring cell growth in shaker flasks commonly used in biological laboratories. The system uses an open-source microprocessor board to monitor concentration of Escherichia coli host cells. To allow measurement for a range of filling degrees and shaker speeds, the backscattering angle is chosen to minimize interference from surface reflections and the measurement window is synchronized to the position of the shaker flask. A nonlinear calibration model of scattered light can predict offline optical density with a mean relative error of 5.2%, an accuracy which is comparable to the classical offline method and sufficient for biotechnology applications.
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KEYWORD
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biomass concentration, optical density, optical sensor, scattered light, shaker flask
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