|
KMID : 1094720170220050518
|
|
Biotechnology and Bioprocess Engineering
2017 Volume.22 No. 5 p.518 ~ p.528
|
|
|
Omega-3 fatty acids concentrate production by enzyme-catalyzed ethanolysis of supercritical CO2 extracted oyster oil
|
|
Lee Hee-Jeong
Haq Monjurul
Saravana Periaswamy Sivagnanam
Cho Yong-Nam
Chun Byung-Soo
|
|
|
Abstract
|
|
|
|
The separation of oil by a suitable technique from the Pacific oyster muscle is important for the utilization of the oil as a ω-3 polyunsaturated fatty acids (ω-3 PUFAs) source and production of bio-functional peptides/ oligosaccharides from oil-free residue. This study was conducted to prepare ω-3 PUFAs concentrate from supercritical carbon dioxide (SC-CO2) extracted Pacific oyster oil by enzyme-catalyzed ethanolysis reactions. SC-CO2 extractions were done at different temperatures and pressures to optimize suitable extraction conditions and extracted oils were compared with Soxhlet (n-hexane) extracted oil to evaluate the yield and quality. Oil extracted by SC-CO2 at optimized conditions was used for ethanolysis reaction catalyzed by immobilized sn-1,3 specific lipases, namely Novozymes-435, Lipozyme TLIM, and Lipozyme RMIM to produce 2-monoacylglycerols (2-MAG) rich in ω-3 PUFAs. The optimum temperature and pressure for SC-CO2 extractions of oyster oil was 50°C and 30 MPa. In this condition, the yield of oil was 5.96% and the acid, peroxide, free fatty acid, and p-anisidine values were 4.49 mg KOH/g, 4.72 meq/kg, 3.42%, and 10.03, respectively. The ω-3 PUFAs content significantly increased in 2-MAG obtained from Novozymes 435, Lipozyme TLIM, and Lipozyme RMIM to 43.03 ± 0.36, 45.95 ± 0.29, and 40.50 ± 0.77%, respectively (p < 0.05). A thin layer chromatography (TLC) analysis confirmed the production and separation of 2-MAG in the ethanolysis process. The ratio of total ω-3 to ω-6 fatty acids was almost twice in 2-MAG of SC-CO2 extracted oyster oil. SC-CO2 extracted Pacific oyster oil can be used for sn-1,3 specific lipases catalyzed ethanolysis to produce ω-3 PUFAs rich in 2-MAG.
|
|
|
KEYWORD
|
|
|
supercritical CO2, pacific oyster oil, ω-3 polyunsaturated fatty acids, ethanolysis, sn-1,3 specific lipase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|