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KMID : 1094720180230010049
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Biotechnology and Bioprocess Engineering
2018 Volume.23 No. 1 p.49 ~ p.54
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A Novel Cell Disruption Approach: Effectiveness of Laser-induced Cell Lysis of Pichia pastoris in the Continuous System
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Gazor Maryam
Talesh Seyed Siamak Ashraf
kavianpour Alireza
Khatami Maryam
Javidanbardan Amin
Hosseini Seyed Nezamedin
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Abstract
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In biotechnological processes, often cell disruption has been an inevitable step as current host cells express most of the desired products intracellularly. Thus, an appropriate cell disruption technique must be selected considering different factors including the target product, process scale, and cell wall structure. In the current study, as a novel method, the efficacy of cell disruption via laser was tested qualitatively and quantitatively in batch and continuous systems, respectively. Laser-induced cell lysis can be a clean, rapid and convenient alternative to the other conventional disruption techniques. Our investigations in the continuous system with a flow rate of 800 μL/sec proved efficient (~ 90%) Pichia pastoris cell disruption at the wavenumber 1,064 nm with the energy input of 284 mW after four complete rounds of circulation. The main mechanism of cell disruption is assumed to be thermolysis via instant heat increase in the laser-treated spot. The results of the current study showed that continuous laser system could be applied in laboratory and industry scale for cell disruption.
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KEYWORD
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laser, cell disruption, continuous and batch systems, Pichia pastoris, recombinant Hepatitis B surface antigen
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