|
KMID : 1094720180230050598
|
|
Biotechnology and Bioprocess Engineering
2018 Volume.23 No. 5 p.598 ~ p.604
|
|
|
Immobilized Lipid Affinity Capture for Antimicrobial Peptides Screening
|
|
Joo Hwang-Soo
|
|
|
Abstract
|
|
|
|
A novel method for rapid screening of antimicrobial peptides (AMPs) was developed by using immobilized lipid affinity capture (ILAC) coupled with LC-MS. Phospholipid (PL) mixture containing phosphatidyl glycerol (PG): phosphatidyl ethanolamine (PE) (4:1), roughly mimic the PL composition of Gram-positive bacterial membrane, was covalently immobilized on magnetic particles (MPs). PL monolayer immobilized on MPs was used as a matrix for capturing of the membrane-disruptive AMPs. Hominicin, a new AMP against Gram-positive bacteria, was successfully captured by ILAC from the peptide pool of Staphylococcus hominis MBBL 2?9. The hominicin was identified by the comparative analysis of LC-MS 2Dprofiles of peptides captured by bare and PL-immobilized MPs. This is the first report for the development of rapid AMP screening method using lipid-immobilized MPs and LC-MS which will be a promising tool for discovery of various kinds of AMPs.
|
|
|
KEYWORD
|
|
|
antimicrobial peptides, affinity capture, phospholipids, magnetic particle, LC-MS
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|