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KMID : 1094720190240050833
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Biotechnology and Bioprocess Engineering
2019 Volume.24 No. 5 p.833 ~ p.838
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Formulation of a Low-cost Medium for Improved Cost-effectiveness of Hydrogen Production by Thermococcus onnurineus NA1
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Lee Sung-Mok
Lee Joung-Min
Lee Seong-Hyuk
Kim Jae-Young
Lee Hyun-Sook
Kang Sung-Gyun
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Abstract
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Hydrogen has been receiving considerable attention as a clean energy carrier. Thermococcus onnurineus NA1, a hyperthermophilic archaeon, is capable of producing hydrogen using carbon monoxide (CO) in a growth-associated manner, providing a promising platform to upcycle industrial waste gases. In order to improve cost-effectiveness of the biological hydrogen production from carbon monoxide (CO), we developed formulation of a low-cost medium for T. onnurineus 156T by substituting fishmeal and sea salt for yeast extract (YE) and reagentgrade salts, respectively. In serum bottle cultures, hydrogen production by the 156T strain was less than 30% in fishmeal-containing media when compared to that of the YE-containing MM1 medium. However, hydrogen production was increased substantially by adaptive evolution in the fishmeal-sea salt (FMSS) medium. In bioreactor experiments, the resulting strain 156T-FA showed shorter lag phase and 70% higher maximum hydrogen production rate in FMSS medium than the parent strain 156T. Our results showed that the H2 production cost was reduced by ca. 95%, when YE and chemical salts were replaced by fishmeal and sea salt, respectively.
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KEYWORD
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hydrogen, carbon monoxide (CO), medium cost, Thermococcus onnurineus NA1, adaptive evolution
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