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KMID : 1094720220270020268
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Biotechnology and Bioprocess Engineering
2022 Volume.27 No. 2 p.268 ~ p.275
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Production of Polyhydroxyalkanoates with the Fermentation of Methylorubrum extorquens Using Formate as a Carbon Substrate
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Chang Woo-Jin
Yoon Ji-Hee
Oh Min-Kyu
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Abstract
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Deriving both carbon and energy from formate, a single-carbon substrate, for the microbial production of value-added products allows its use as the main feedstock in biorefinery, with consequent environmental and economic benefits. Methylorubrum extorquens AM1, a strain capable of growth solely on formate, is a known producer of the biopolymer polyhydroxyalkanoate (PHA), during nitrogen-deficient growth. Based on findings from our previous report, the gene ftfL was selected for overexpression to enhance growth and PHA production using formate. Its overexpression in a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)-producing strain yielded improved growth and a 1.4-fold increase in PHBV production. The strain could thus be tested for long-term fermentation, intended for optimized growth and production in formate. A customized fermentation regimen was established by incorporating both the conventional two-phase fermentation method for PHA production and a repeated fed-batch fermentation process designed to resolve the problem of sodium accumulation. The poly-3-hydroxybutyrate and PHBV copolymer-producing strains resulted in 11.07 g/L and 2.76 g/L of bio-degradable polymers, respectively, in the fermentation process.
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KEYWORD
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Methylorubrum extorquens, formate, polyhydroxyalkanoate, repeated fed-batch fermentation
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