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KMID : 1094720220270030415
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Biotechnology and Bioprocess Engineering
2022 Volume.27 No. 3 p.415 ~ p.422
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Improved Extracellular Enzyme-mediated Production of 7,10-dihydroxy-8(E)-octadecenoic Acid by Pseudomonas aeruginosa
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Jeong Ji-Wan
Singhvi Mamata
Kim Beom-Soo
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Abstract
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In this study, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) was produced using extracellular enzymes present in the supernatant of Pseudomonas aeruginosa. DOD concentration and productivity were improved using extracellular enzymes obtained from P. aeruginosa culture broth than using conventional whole cell culture method. Among the different carbon sources used during cell pre-culture, glycerol showed higher DOD production than using glucose or crude glycerol. In batch process, the highest concentration of DOD (8.82 g/L) was accomplished using supernatant derived from 12 h-grown pre-culture of P. aeruginosa strain after 72 h of bioconversion process. To further improve DOD production, a fed-batch process was used with the addition of surfactant and enzyme concentrate. The fed-batch process using a 4-fold concentrated enzyme solution with Tween 80 yielded the highest DOD concentration (27.5 g/L at 72 h) with an 8.28-fold increase in DOD production compared to using whole cells. The prominence of this study was that the highest DOD production (27.5 g/L) ever reported was achieved using extracellular enzymes via a fed-batch process. Another significant aspect of this study is the possibility of utilizing abundantly available crude glycerol substrate for DOD production, which will help direct the development of sustainable process. Therefore, the overall study is projected to speed up the development of DOD production using inexpensive substrates such as crude glycerol on a larger scale.
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KEYWORD
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7,10-dihydroxy-8(E)-octadecenoic acid, Pseudomonas aeruginosa, extracellular enzyme, oleic acid, fed-batch process
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