|
KMID : 1094720220270040607
|
|
Biotechnology and Bioprocess Engineering
2022 Volume.27 No. 4 p.607 ~ p.614
|
|
|
Affinity Peptide-based Electrochemical Biosensor for the Highly Sensitive Detection of Bovine Rotavirus
|
|
Cho Chae-Hwan
Park Tae-Jung
Park Jong-Pil
|
|
|
Abstract
|
|
|
|
Bovine diarrhea is a major concern in the global bovine industry because it can cause significant financial damage. Of the many potential infectious agents that can lead to bovine diarrhea, bovine rotavirus (BRV) is a particular problem due to its high transmissibility and infectivity. Therefore, it is important to prevent the proliferation of BRV using an early detection system. This study developed an affinity peptide-based electrochemical method for use as a rapid detection system for BRV. A BRV-specific peptide was identified via the phage display technique and chemically synthesized. The synthetic peptide was immobilized on a gold electrode through thiol-gold interactions. The performance of the BRV specific binding peptides was evaluated using square wave voltammetry. The developed detection system exhibited a low detection limit (5 copies/mL) and limit of quantitation (2.14 × 102 copies/mL), indicating that it is a promising sensor platform for the monitoring of BRV.
|
|
|
KEYWORD
|
|
|
bovine diarrhea, bovine rotavirus, phage display, affinity peptide, electrochemical sensor
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|