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KMID : 1100720150350040423
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Annals of Laboratory Medicine
2015 Volume.35 No. 4 p.423 ~ p.428
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Comparison of FcRγ-Deficient and CD57+ Natural Killer Cells Between Cord Blood and Adult Blood in the Cytomegalovirus-Endemic Korean Population
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Baek Hee-Jo
Kim Da-Woon
Phan Minh-Trang Thi
Kim Ju-Sun
Yang Ji-Hoon
Choi Jeong-Il
Lee Je-Jung
Shin Myung-Geun
Ryang Dong-Wook
Kim Sang-Ki
Lee Seung-Hwan
Kook Hoon
Cho Duck
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Abstract
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Background: FcRγ-deficient natural killer (NK) cells (g?NK cells) have been associated with cytomegalovirus (CMV) infection. However, the frequency of g?NK cells in a CMV-endemic area (i.e., Korea) has not yet been studied. We examined the frequency of g?NK cells and expression of CD57 on NK cells in cord blood (CB) and adult blood (AB).
Methods: Of the 24 AB samples collected, 95.8% (23/24) were CMV IgG+/IgM-, while 100% of the 13 healthy CB samples were CMV IgG+/IgM-. We performed whole-blood flow cytometry assays to analyze intracellular FcRγ and CD3ζ expression of CD3-/CD56dim NK cells from 13 CB and 24 AB samples, and surface CD57 expression on CD3-/CD56dim/CD16+ NK cells from 13 CB and 19 AB samples.
Results: All CMV seropositive AB samples contained g?NK cells (23/23), and the median proportion of g?NK cells in the CD3-/CD56dim NK cell pool was 35.0% (range: 11-77%). CD57+ NK cells in the CD3-/CD56dim/CD16+ NK cell population were detected in all 19 AB samples tested, but not in any CB samples.
Conclusions: Our data suggest that g?NK cells and CD57+ NK cells are present at a very high frequency in CMV-seropositive AB, but rare in CMV-naive CB.
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KEYWORD
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Adult, Blood, Cord, Cytomegalovirus, Infection, FcRγ, Natural killer cell
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