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KMID : 1100720190390060566
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Annals of Laboratory Medicine
2019 Volume.39 No. 6 p.566 ~ p.571
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Comparison of Six Commercial Diagnostic Tests for the Detection of Dengue Virus Non-Structural-1 Antigen and IgM/IgG Antibodies
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Lee Hye-Young
Ryu Ji-Hyeong
Park Hye-Sun
Park Ki-Hyun
Bae Hyun-Joo
Yun So-Jeong
Choi Ae-Ran
Cho Sung-Yeon
Park Chul-Min
Lee Dong-Gun
Lim Ji-Hyang
Lee Je-Hoon
Lee Seung-Ok
Shin So-Young
Park Hae-Il
Oh Eun-Jee
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Abstract
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ELISAs and rapid diagnostic tests (RDTs) are widely used for diagnosing dengue virus (DENV) infection. Using 138 single blood samples, we compared the ability to detect non-structural (NS)-1 antigen and anti-DENV IgM/IgG antibodies among (1) DENV Detect NS1 ELISA, DENV Detect IgM capture ELISA and DENV Detect IgG ELISA (InBios International, Inc.); (2) Anti-Dengue virus IgM Human ELISA and Anti-Dengue virus IgG Human ELISA (Abcam); (3) Dengue virus NS1 ELISA, Anti-Dengue virus ELISA (IgM) and Anti-Dengue virus ELISA (IgG) (Euroimmun); (4) Asan Easy Test Dengue NS1 Ag 100 and Asan Easy Test Dengue IgG/IgM (Asan Pharm); (5) SD BIOLINE Dengue Duo (Standard Diagnostics); and (6) Ichroma Dengue NS1 and Ichroma Dengue IgG/IgM (Boditech Med). For NS1 antigen detection, InBios and Euroimmun showed higher sensitivities (100%) than the RDTs (42.9?64.3%). All tests demonstrated variable sensitivities for IgM (38.1?90.5%) and IgG (65.7?100.0%). InBios and Boditech Med demonstrated higher sensitivity (95.6% and 88.2%, respectively) than the other tests for combined NS1 antigen and IgM antibody. Five NS1 antigen tests had good agreement (92.8?98.6%) without showing positivity for chikungunya. However, all IgG tests demonstrated potential false-positivity with variable ranges. Clinical laboratories should note performance variations across tests and potential cross-reactivity.
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KEYWORD
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Dengue virus, Diagnosis, Rapid diagnostic tests, ELISA, Performance
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